Variability of allergen – based length polymorphism of Glycine max L. varieties

Legumes belong to nutritionally attractive crops, because of their high protein content and very well-balanced nutritional value. However, in addition to nutritionally valuable components, they contain a relatively high amount of anti-nutritional factors such as glycosides, lectins, inhibitors of digestive enzymes, and anti-nutritional proteins, what include allergens as well. Different genomic based analysis of allergen coding parts are relevant in the research of legume gene resources. Here, a total of twenty nine different soybean (Glycine max L.) varieties were analysed for their polymorphism based in the specific homologous sequences of genes for vicilin and profilin, products of both belong to allergenic molecules of this specie. A total of 16 different amplicons were obtained when profilin was used as marker and 17 different amplicons for vicilin.
Comparing both of used techniques, vicilin provided more polymorphic profiles, but in five out of the analysed verieties no amplicons were obtained. Profilin fingerprints provided higher degree of similarity coefficients among individual varieties of soybean. Both of used PCR based techniques were proved to be applicable for genomic based screening of allergen homologs in the genetic resources of Glycine max L.