Events The 4th International Electronic Conference on Synthetic Organic Chemistry

Event submissions

Published

This submission belongs to the session b. Solid Phase Chemistry and Combinatorial Synthesis of the event The 4th International Electronic Conference on Synthetic Organic Chemistry

Published date

11 Sep, 2000

Citation

Markus Kunz, Peter A. Lohse, Guannan Kuang, An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols, in Proceedings of The 4th International Electronic Conference on Synthetic Organic Chemistry, 1 November–30 November 2000, MDPI: Basel, Switzerland, doi: 10.3390/ecsoc-4-01892

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An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols

Peter A. Lohse ¹

Guannan Kuang ¹

Markus Kunz ¹

1. Phylos, Inc., 128 Spring Street, Lexington, MA 02421

Abstract

We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the preparation of fusion libraries and should prove most useful for in vitro protein evolution procedures which involve repetitive cycles of fusion library preparation and selection.

Keywords

Nucleic acid-encoded libraries

mRNA-protein fusion

PROfusionTM

in vitro selection

puromycin

psoralen.

Dendrimers as Scavengers for Rapid Purification in Solution-Phase Parallel Synthesis

Solid-Phase Synthesis of Peptides, Peptidomimetics, and Cyclic Peptides Using Traceless Aromatic Side-Chain Tethered Building Blocks