A phytochemical, antioxidant and anti-inflammatory study of extracts of the Appiaceae family and their fraction. “study of the effect of synergy and/or antagonism”

The purpose of this work was to study the phytochemical screening, antioxidant and anti-inflammatory activity of extracts from the fruit of a Saharan plant of the Appiaceae family as well as their fractions. An extensive exploration is undertaken to investigate the phytochemical composition of extracts, with a particular focus on alkaloids, tannins, flavonoids, coumarins, and saponins. Phytochemical screening revealed the presence of alkaloids, tannins, and flavonoids within extracts, suggesting a rich reservoir of bioactive compounds. Quantification assays further confirmed the abundance of polyphenols and flavonoids, indicative of the extract's potential health-promoting properties. The result of the evaluation of the antioxidant activity revealed that the methanoic extract is endowed a very powerful antioxidant activity characterized by inhibitory concentrations of 50% of the DPPH free radical (IC50 = 0.2 mg/mL), which is better than that of BHT (IC50 = 0.5 mg/mL). While for anti-inflammatory activity, the results revealed that the methanoic extract has an activity (IC50 = 42.3 µg/mL) higher than that of diclofinac (IC50 = 83.3 µg/mL). Fractionation of the extract via column chromatography allowed for the isolation of distinct fractions, enhancing the concentration of bioactive constituents.In fact, these new findings could lead to powerful approaches for the development of new antioxidant and anti-inflammatory compounds.