Green Synthesis of Silver Nanoparticles Using Phyllanthus emblica and Adhatoda vasica Leaf Extract and their comparative study on microbes.

INTRODUCTION

Silver played an important role as a novel metal ion in curing many diseases and infections. Silver is used in the form of AgNPs for targeting many biomedical and ,physio-chemical reactions to fulfill research goals. However, many drawbacks are reported in AgNP reactions, such as allergy and environmental risks. Therefore, to avoid all these side-effects, plant-based AgNPs are synthesized. In our research, we have used silver nano-particles from P.emblica and A.vasica leaf extract and carried out a comparative study on microbes.

METHODS

Leaves were first collected and then crushed into a powder. Next, we made a water-based extract solution by heating the mixture to 80 degrees Celsius for three to four hours using a magnetic stirrer. The leaf extract was combined with 1M silver nitrate solution, made by dissolving 1.7 grams of silver nitrate in 100 milliliters of water. P. emblica and A. vasica Leaf Extract with silver nitrate solution was centrifuged at 12000 rpm for 30 minutes, discarding the supernatant and collecting the dark pellet to form AgNPs from the leaf extract. Finally, the leaf extract was collected in the form of a powder and dried for two to three days in a dark place. Using the disc diffusion and well diffusion methods, we investigated the effects of these AgNPs powders at varying concentrations against bacteria that cause disease, such as E. coli, S. aureus, Mucor, and Aspergillus strains. Additionally, we utilized the commercial antibiotic streptomycin to complete a comparative study.

CONCLUSIONS

Secondary metabolites in plant leaves makes plant- based drug systems and P.emblica and A.vasica Leaf Extract AgNPs molecules more effective and eco-friendly as compared to chemical-based AgNPs.

RESULT

In our research, a comparative study of the effects of P. emblica and A. vasica Leaf Extract AgNPs on microbes produced positive results as compared to the commercial antibiotic streptomycin.