An emerging high-throughput biosensing platform for membrane interaction studies

This presentation highlights the use of an emerging electrochemical biomembrane sensor for the (bio)membrane activity assessment of nanomaterials/advanced materials. It utilizes a self-assembled dioleoyl phosphatidylcholine (DOPC) monolayer on a fabricated microHg-on-Pt chip electrode to generate characteristic rapid cyclic voltammograms (RCV) at 40 Vs^-1. These RCVs contain current peaks due to underlying phase transitions in response to the applied electric field. Changes in the RCV scan and associated capacitance peaks in the presence of (bio)membrane active substances are related to membrane disruption, detailing the nature and extent of the interactions. These interactions will be related to the MIE (molecular initiating event) of each species, providing insight into the mechanism of their interaction with the biomembrane-like sensor layer. Extracting membrane affinity parameters from the data enables the estimation of the structure–activity relationships (SARs) of the materials with the sensing layer. The biomembrane sensor has successfully been intercalibrated with in vitro analysis through MTT assays using colchicine, methyl methane sulfonate and chlorpromazine and exhibited more than ten times higher sensitivity. This unique advanced material screening technology, the results and their analysis will be presented at this conference.

The BIO-SUSHY project is funded by the European Union under Grant Agreement Number 101091464. The University of Leeds is funded by the UKRI Horizon Europe Guarantee Fund, Grant Number 10056199. The views and opinions expressed are, however, those of the author(s) only and do not necessarily reflect those of the European Union or the European Health and Digital Executive Agency (HaDEA).