Antibacterial, antiobesity, and toxicity effects of cherry stem ethanolic extract

Due to their increasing prevalence, obesity and infectious diseases represent major public health problems, which is why effective therapeutic alternatives need to be found. There is considerable interest in the potential health benefits of cherry stems, a by-product of the agri-food industry. In this work, we studied the impact of the ethanolic extract of cherry stems on the inflammation and oxidative stress associated with obesity, as well as its antibacterial effect. In addition, acute oral toxicity was assessed in female BALB/c mice. Furthermore, the quantity of bioactive substances was estimated in the cherry stems extract. To do this, a phytochemical analysis was carried out, then the biological activities related to obesity reduction were examined in vitro and in vivo. The effect of the extract on several pathogens was also assessed, as well as its synergy with antibiotics. The in vitro studies have shown significant amounts of vitamin C and phenolic compounds (3.93 mg ASE/g and 215.52 ± 2.46 mg GAE/g extract, respectively). At a concentration of 01 mg/ml, the extract showed a significant anti-inflammatory (67.11%), antioxidant (60.17 and 64.23%) and anti-obesity (28.29 and 76.66%) properties. The acute oral toxicity of crude ethanolic cherry stem extract, even at high doses, revealed no observable toxicity. The data indicate that cherry extracts exhibit significant antimicrobial activity, with growth inhibition levels ranging from 10 to 98.50%. This is mainly attributed to their bioactive composition. When this extract is combined with antibiotics, they enhance the inhibition of bacterial proliferation, showing an encouraging synergistic effect. In addition, this plant has demonstrated bactericidal action (66.66 - 77.77%) against various strains of pathogenic bacteria. All these factors suggest that cherry stems could be a natural alternative for combating bacterial infections and obesity and their related disorders.