In Vitro Antioxidant study of Colebrookea oppositifolia Sm. extract for treatment of Alzheimer’s Disease

Background: Dementia is a major cause of dependency, disability, and mortality, characterized by a progressive cognitive decline that makes daily tasks difficult. Alzheimer's disease, a major neurodegenerative dementia, primarily affects the elderly population. Early identification and the usage of natural plant-based phytoconstituents may lower the risk and delay the advancement of the condition, even though there are presently no disease-modifying medications available.

Aim(s): This research aimed to find out the antioxidant and neuroprotective potential of plant-derived phytoconstituents for the treatment of Alzheimer’s disorder.

Methods: The Soxhlet extraction method was used to isolate the primary phytoconstituent from the plant (Colebrookea oppositifolia Sm.) using its aerial and root parts. The particular extraction technique used complies with the requirements as stated. The antioxidant potential of the plant phytoconstituent was then assessed using an in vitro antioxidant assay.

Results: The percentage yield for the plant extract carried out by using the hot continuous percolation technique (Soxhlet Extraction Method). In comparison to the aerial parts (13.80% w/w), the root extract (14.10% w/w) was found to have a higher percentage yield. During the in vitro analysis, the root extract showed higher antioxidant potential compared to the aerial extract.

Conclusion: The plant extract (root) showed significant antioxidant potential based on preliminary results, and it was chosen for further thorough research including the in vivo studies (animal study). To fully investigate its potential medicinal uses, more research is necessary, which is in a continuous phase.