CAN A CLAY MATERIAL (GREEN CLAY FROM ANYAMA) IMPROVE BONE HEALING? STUDY BY DETERMINING THE ALKALINE PHOSPHATASE LEVEL IN WISTAR RATS

Introduction:

Implantology has revolutionized dentistry, and we are therefore witnessing a renewed interest in biomaterials inserted into the alveolar bone. Anyama is a city in Ivory Coast. Alkaline phosphatase is used as a marker of bone activity during bone healing.

The objective was to analyze the effects of Anyama green clay (AVA) on the alkaline phosphatase level during the healing of the alveolar bone of Wistar rats.

Methods:

Bone defects were created in 36 Wistar rats. The wounds were treated or not (control rats) with fine fractions of AVA (particle diameter < 2 µ) at different pH levels (acidic, neutral, basic) in dry or paste form. Blood samples were collected on D0, D15, and D30 from the tail for the measurement of alkaline phosphatase.

A Kruskal-Wallis rank test was used to analyze the results.

Results:

The dry form of AVA causes an increase in alkaline phosphatase activity from day 0 to day 15 and stabilizes between day 15 and day 30, except at an acidic pH where we observed a strong increase from day 0 to day 30.

The acidic paste clay seemed to cause an abnormal increase in ALP levels.

However, there was no significant correlation between the pH of the clay and the fluctuations in alkaline phosphatase levels (p-value = 0.2268).

Conclusion:

Within the limits of this study, the dry form of AVA at basic pH could promote good bone formation.

The measurement of PAL must be completed by histological studies.