The deterioration of fluvial systems represents an environmental problem associated with urban growth, agricultural activities, and industrial development, which promote the dispersion of contaminants within hydrographic watersheds. In this context, identifying environmentally vulnerable areas is a useful tool for strengthening integrated pollution management strategies. This study aimed to assess the environmental vulnerability of the Grande Tulancingo River watershed using a multicriteria geospatial analysis that integrates biophysical and anthropogenic variables. The hydrographic delimitation of the watershed was carried out by processing an INEGI Digital Elevation Model using the SIATL simulator, allowing the definition of the drainage network, the main channel, and the catchment area. Biophysical and anthropogenic variables were integrated and processed using Geographic Information Systems (ArcGIS 10.8). Criteria selection, standardisation, reclassification, and weighting were conducted in accordance with specialised scientific literature. Subsequently, the criteria were integrated using the Weighted Overlay method to generate an environmental vulnerability map classified into five categories. The results indicated that the watershed covers an area of 1,887.623 km² and exhibits distinct hydrographic dynamics among the upper, middle, and lower zones, favouring contaminant transport toward accumulation areas. Agricultural activities cover approximately 58.2% of the watershed and were identified as the primary potential source of diffuse pollution from fertilisers and pesticides. The multicriteria analysis showed that 16.6% of the watershed exhibits moderate to very high environmental vulnerability, primarily in areas near the main channel and in zones with high hydrological connectivity. The multicriteria geospatial approach allowed the identification of priority areas for environmental monitoring and management, strengthening decision-making processes aimed at protecting aquatic ecosystems.