EventsMOL2NET'15, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 1st ed.
Event submissions
Published
with-doi10.3390/MOL2NET-1-b035
This submission belongs to the session 02. NANOBIO.MAT-01: NANOBIO.MAT-09: Nanotechnology, Biomed. Eng., & Materials Science Congress, Jackson & Fargo, USA, 2015. of the event MOL2NET'15, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 1st ed.
Published date
04 Dec, 2015
Citation
Hasna Ahyayauch, Jesús Sot, Felix M. Goni, Alicia Alonso, Marta G. Lete, Histones Bind, Aggregate and Fuse Phosphoinositides Containing Bilayers, in Proceedings of MOL2NET'15, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 1st ed., 5 December–15 December 2015, MDPI: Basel, Switzerland, doi: 10.3390/MOL2NET-1-b035
Share
Email
Facebook
Twitter
LinkedIn

Histones Bind, Aggregate and Fuse Phosphoinositides Containing Bilayers

Marta G. Lete 1
Hasna Ahyayauch 2
Jesús Sot 1
Felix M. Goni 1
Alicia Alonso 1
1. Unidad de Biofísica (CSIC, UPV/EHU) and Departamento de Bioquímica, Universidad del País Vasco, Leioa, Spain
2. Unidad de Biofísica (CSIC, UPV/EHU) and Departamento de Bioquímica, Universidad del País Vasco, Leioa, Spain; Institut de Formation aux Carrieres de Sante de Rabat (IFCSR), Rabat, Morocco
Abstract

Recently the presence of phosphoinositides (PIPns) in the eukaryotic cell nucleoplasm and nuclear membrane has been described (1). We have studied the interaction of the nuclear proteins, histones, with these lipids in model membranes. Turbidimetric studies revealed that histones induce extensive aggregation of vesicles when containing phosphatidylinositol-4-phosphate (PtdIns(4)P) and in a smaller extent when containing phosphatidylinositol (PtdIns). Binding and aggregation events were visualized by confocal microscopy. Using isothermal calorimetry we determined that the binding of histone H1 to PtdIns(4)P was one order of magnitude higher than with PtdIns.

Intervesicular mixing of total lipids and inner monolayer lipids was found in vesicles containing PtdIns(4)P, but not on those containing PtdIns. Supporting our hypothesis about the role histones could play promoting fusion events.

Taking this together we suggest that a complex interplay of histones and phosphoinositides could be taking place in the nucleoplasm where histones could have an important role during the remodeling processes of the mitotic cell cycle.

 

  1. Byrne, R. D., M. Garnier-Lhomme, K. Han, M. Dowicki, N. Michael, N. Totty, V. Zhendre, A. Cho, T. R. Pettitt, M. J. Wakelam, D. L. Poccia, and B. Larijani. 2007. PLCgamma is enriched on poly-phosphoinositide-rich vesicles to control nuclear envelope assembly. Cellular signalling 19:913-922.

 

Keywords
Histones
Phosphoinositides
Bilayers
Manuscript
6-Comunication_Marta_G_Lete.pdf
Uptake of different organic pollutants by carrot
New theoretical model for the study of new β-secretase inhibitors