Bioprinting complex human tissues

3D bioprinting can create living human tissues on demand based on specifications contained in a digital file. One of the benefits of using this additive manufacturing technique is the ability to generate complex constructs containing a variety of cells that mimic structures found in the human body. Such highly customized, physiologically relevant 3D human tissue models can screen potential drug candidates as an alternative to expensive pre-clinical animal testing as well as hold the potential to regenerate diseased and damaged tissues. Over the past few years, the Willerth lab has been a leader in the 3D bioprinting space. The Willerth lab has developed a novel fibrin-based bioink called TissuePrint for bioprinting human tissues that has been commercialized through her award-winning spin-off company – Axolotl Biosciences. We have developed several tissue models using TissuePrint, including constructs derived from human induced pluripotent stem cells (hiPSCs), which can become any cell type found in the body. More recent work has focused on developing our “smart” bioinks that contain drug releasing microspheres produced using microfluidics as a way to improve the reproducibility of the bioprinting process. Using our novel, patent-pending BrainPrint bioink, we have generated tissues with chemical and electrical properties similar to that of the brain and spinal cord.