The Niger Delta region of Nigeria is the main centre for oil and gas operations, many of which overlap with ecologically sensitive areas and critical habitats. Identifying critical habitats and their distribution across the Niger Delta region is essential for improving biodiversity protection within Environmental Impact Assessments (EIAs). This study applied the International Finance Corporation's Performance Standard 6 (IFC PS6) to systematically identify and map critical habitats across the Niger Delta. IFC PS6 recognises protected areas (PAs) and internationally recognised areas (IRAs), including Ramsar wetlands, United Nations Educational, Scientific and Cultural Organization (UNESCO) Man and the Biosphere (MAB) Reserves, Natural World Heritage Sites, and Key Biodiversity Areas (KBAs) as areas that may qualify as critical habitats. The spatial datasets of these areas were integrated within a GIS environment to identify their distribution within the Niger Delta region. The analysis identified 24 critical habitats within the Niger Delta comprising 4 Ramsar sites, 9 KBA, 3 UNESCO MAB Reserves and 22 International Union for the Conservation of Nature (IUCN) Legally Protected Areas, with several sites holding multiple conservation designations. Spatial evidence indicates that 10 of these habitats may be under threat from oil and gas activities highlighting areas where biodiversity may be at greatest risk. The resulting map provides a regional decision-support tool for project screening, EIA preparation, biodiversity conservation and environmental planning. The findings demonstrate how IFC PS6 can be applied systematically to strengthen biodiversity conservation and support more sustainable oil and gas development in Nigeria.