EventsMOL2NET'16, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 2nd ed.
Event submissions
Published
with-doi10.3390/mol2net-02-03883
This submission belongs to the session 01. CHEMBIOINFO-02: Chem-Bioinformatics Congress Cambridge, UK-Chapel Hill and Richmond, USA, 2016. of the event MOL2NET'16, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 2nd ed.
Published date
20 Jan, 2017
Citation
Verónica López Carbón, Ana Sayago, Ángeles Fernández-Recamales, Raúl González-Domínguez, Rafael Beltrán, DEVELOPEMENT AND OPTIMIZATION OF ACALORIC SWEETENERS EXTRACTION PROCEDURE FROM Stevia rebaudiana Bertoni, in Proceedings of MOL2NET'16, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 2nd ed., 15 October–20 October 2022, MDPI: Basel, Switzerland, doi: 10.3390/mol2net-02-03883
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DEVELOPEMENT AND OPTIMIZATION OF ACALORIC SWEETENERS EXTRACTION PROCEDURE FROM Stevia rebaudiana Bertoni

Verónica López Carbón 1
Ángeles Fernández-Recamales 1
Ana Sayago 1
image
Raúl González-Domínguez 1
Rafael Beltrán 1
1. Dpto. Química "Prof. J.C. Vilchez Martín", Facultad de Ciencias Experimentales, Universidad de Huelva, Campus de «El Carmen», Campus de Excelencia Internacional Agroalimentario (CEIA3), Avda. de las Fuerzas Armadas, S/N. 21071 Huelva
Abstract

Stevia rebaudiana Bertoni is a shrub of the Asteraceae family native to South America, especially of Brazil and Paraguay. This plant accumulates a suite of diterpenoid metabolites that are natural sweeteners finding increased use as sugar. In addition, a number of healing properties have been associated to the stevia leaves intake.

The objectives of the present work have been to develop an optimum extraction method of the main steviol glycosides, stevioside and rebaudioside A, from the Stevia leaves studying the influence of the several factors that affect the process. Applying experimental design methodology, parameters such as, the grinding of the leaves, the temperature and time of extraction, the agitation of the process, the quantity of dried leaves and the volume of dissolvent were assessed. In order to eliminate the impurities and to obtain a crystalline white precipitate several procedures were tested.

Moreover, a HILIC chromatographic method to identify and quantify steviol glycosides from stevia leaves were optimized.

Keywords
Stevia rebaudiana
steviol glycosides
HILIC
Poster
DEVELOPMENT AND OPTIMIZATION OF ACALORIC SWEETENERS EXTRACTION PROCEDURE FROM Stevia rebaudiana Bertoni (1).pdf
DEVELOPMENT AND OPTIMIZATION OF ACALORIC SWEETENERS EXTRACTION PROCEDURE FROM Stevia rebaudiana Bertoni (1).pdf
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