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This paper presents a novel chemical sensor using a metamaterial absorber, which is composed of an Au Bottom layer, a microfluidic channel, a FR4 substrate and a split-square-cross-resonator (SSCR). The resonance generated by SSCR is extraordinarily sensitive to changes of the effective dielectric constant around the capacitive gap. Furthermore, the effective dielectric constant of the dielectric substrate is under the influence of microfluidic channels by using an infinitesimal quantity of a liquid. The proposed sensor exhibits an outstanding sensitivity by a creative SSCR structure and the Au Bottom layer. In addition, the relationship between the absorption frequency and chemical concentration is demonstrated by simulation.