Nanofluidics for Single-Cell Proteomics with Single-Molecule Sensitivity

Single cell omics analysis aims to collect all molecule information in single cells in the human body, but still lacks appropriate and efficient tools. Nanofluidics is promising for single cell omics analysis at the single molecule level. However, many challenges have impeded the nascent nanofluidics to enable the goal. A most critical one is the lack of methodology for the high-throughput capture and detection of single molecules in tiny nanofluidic channels. By using the nano-in-nano integration technology which previously developed by us [1-4], we established a nanofluidic nanoarray methodology allowing the high-throughput capture and detection of single molecules in samples with single cell volumes. In this presentation, the nanofluidic nanoarrays for single-cell omics with single-molecule sensitivity will be presented.

[1] Xu Y., et al., Lab Chip, 2015, 15, 1989-1993.
[2] Xu Y., et al., Lab Chip, 2015, 15, 3856-3861.
[3] Xu Y., et al., Small, 2015, 11, 6165-6171.
[4] Xu Y., et al., Adv. Mater., 2016, 28, 2209-2216.