APPLICATION OF COMMERCIAL WHEY PROTEINS FOR EXTRACTION OF BIOACTIVE COMPOUNDS FROM ELDERBERRY

Recently, interest in food of plant origin and its pro-health properties have increased. Plant products due to significant antioxidant activity should be a constant part of the human diet. Elderberry is a plant with high potential as a source of functional component obtained from flowers, fruits, bark and roots.  Wild elderberry flowers are used as a antipyretic, diuretic and sealing blood vessels. On the other hand elderberry fruits are known as the source of many substances that have detoxifying, antiviral and strengthening effects on human body. Outside of therapeutic significance both fruits and flowers are used increasingly in the food industry. Micelle-mediated separation is similar to traditional liquid-liquid extraction. In this method the organic solvent is replace with an aqueous surfactant solution. Micelles are colloidal type cluster that are spontaneously formed when the concentration of surfactant molecules increase above called critical micellar concentration (cmc).The aim of this work was to extract bioactive components from elderberry (Sambucus nigra) fruits and flowers by means of various extraction solutions. The extraction solution were WPC (whey protein concentrate) and WPI (whey protein isolate) solution. Comparative extracts were also made using ethanol solutions and distilled water as control. Obtained extracts were analyzed by determination of: total reduction capacity using Folin-Ciocalteu reagent, flavonoid content, antioxidant activity using DPPH reagent. In WPC and WPI protein extracts, the extraction of bioactive components is more efficient than in the case of water extraction. This could be seen at the lowest concentration of both WPI and WPC. The work was financed by scientific grant
No. DS3700/WTZ/2017