Microwave assisted synthesis and its cytotoxicity study of 4H-pyrano[2,3-a]acridine-3-carbonitrile intermediate: Experiment design for optimization using Response Surface Methodology

Several synthetic routes have been achieved to synthesize pyrane fused systems. As a follow up of earlier work, we hereby report the microwave assisted synthesis of key intermediate, pyrane fused acridine compounds 3a-f. It was obtained by treating α, β- unsaturated ketone 1a-f and malononitrile 2 in presence of piperidine with ethanolic solution at 50 °C under 200 W power. This method also optimized via microwave method using RSM methodology. Among the synthesized derivatives dimethoxy substituted pyrane fused acridine 3b was used as an intermediate for the synthesis of various heterocyclic molecules 4a-e. All the synthesized derivatives and target compounds were evaluated for cytotoxicity effect on human hepatoblastoma (HepG2) cell line and HDAC enzyme activity.