EventsThe 23rd International Electronic Conference on Synthetic Organic Chemistry
Event submissions
Published
with-doi10.3390/ecsoc-23-06698
This submission belongs to the session B. Bioorganic, Medicinal and Natural Products Chemistry of the event The 23rd International Electronic Conference on Synthetic Organic Chemistry
Published date
18 Nov, 2019
Citation
Sandeep Waghulde, Ashwini Bhoir, Jyoti Gholap, Varsha Patil, Mohan Kale, Vijay R. Patil, Cumulative effect of the aqueous and ethanolic extracts of Annona reticulate and Allium sativum on Brine Shrimp Lethality Assay, in Proceedings of The 23rd International Electronic Conference on Synthetic Organic Chemistry, 15 November–15 December 2019, MDPI: Basel, Switzerland, doi: 10.3390/ecsoc-23-06698
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Cumulative effect of the aqueous and ethanolic extracts of Annona reticulate and Allium sativum on Brine Shrimp Lethality Assay

Sandeep Waghulde 1
Ashwini Bhoir 2
Jyoti Gholap 3
Varsha Patil 2
Mohan Kale 4
Vijay R. Patil 5
1. Research Student, Hon’ble Loksevak Madhukarrao Chaudhari College of Pharmacy, Faizpur, Dist: Jalgaon; Maharashtra, India
2. Reserach Scholar, Konkan Gyanpeeth Rahul Dharkar College of Pharmacy and Research Institute, Karjat University of Mumbai
3. Research Scholar, Kokan Gyanpeeth Rahul Dharkar College of Pharmacy & Research Institute, Karjat University of Mumbai
4. Principal & Professor, Konkan Gyanpeeth Rahul Dharkar College of Pharmacy and Research Institute, Karjat, Dist-Raigad University of Mumbai
5. Principal and Professor, Hon'ble Loksevak Madhukarrao Chaudhari College of Pharmacy, Faizpur, Dist: Jalgaon
Abstract

The present study was conducted to test for in vivo Brine Shrimp Lethality Assay (BSLA) of the Aqueous and ethanolic extracts Annona reticulate Linn. and Allium sativum and correlate cytotoxicity results with known pharmacological activities of the plants. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). Ten nauplii were added into three replicates of each concentration of the plant extract. After 24 hours the surviving brine shrimp larvae were counted and LC50 was assessed. Results showed that the presence of alkaloids, tannins, and flavonoids could be accounted for its cytotoxic properties. In the other hand, studies have shown that the leaf extracts of Alcoholic and aqueous extract of Annona reticulata and bulbs of Allium sativum extracts exhibited cumulative activity when they were combined and compared. Thus, the results on the leaf extracts of Alcoholic and aqueous extract of Annona reticulata and bulbs of Allium sativum exhibited increase in activity support its use in traditional medicine.

Keywords
Brine shrimp lethality assay
Annona reticulata
Allium sativum
LC50
potent
cytotoxicity
Manuscript
Cumulative effect of the aqueous and ethanolic .pdf
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