Carbon nanoparticles are known because of their highly fluorescent property. Thus, among several different types of nanoparticles, carbon nanoparticles have great potential of bio imaging applications. Highly fluorescent crystalline carbon nanoparticles (CNPs) have been synthesized in a facile, rapid method which involves microwave irradiation of sucrose with phosphoric acid at 100 W for 4 mins. Hence this method is fleet and cost effective for large scale applications. Physical characterization of synthesized CNPs was done by DLS and Zeta potential, Hydrodynamic size of CNPs as measured by DLS was 281.2 d.nm. The surface charge of carbon nanoparticles was found to be -39.7 mV. These CNPs have green fluorescence under UV exposure. CNPs enter into cell without any further modification and show their efficiency as fluorescence based cell imaging application. Further, we have explored the antibacterial property of carbon nanoparticles by Minimum Inhibitory Concentration (MIC).