Elacteriospermum tapos ameliorates maternal obesity effect on serum leptin changes in male offspring

The purpose of this study is to investigate the effect of Elateriospermum tapos aqueous extract supplementation on serum leptin of male offspring at weaning. Total 30 female Sprague Dawley rats were assigned to 2 groups where control group (CG) consist of 6 rats and the remaining rats were induced obesity for five weeks with a high-fat diet pellet and cafeteria food. After five weeks, the obese group further divided into four groups, negative control group (NG), positive control group (PG) (orlistat 200mg/kg), treatment 1 (TX1) (200mg/kg E.tapos seed) and treatment 2 (TX2) (200 mg/kg E.tapos shell) for 6 weeks. After six weeks, all rats were mated and continue with their respective diet till weaning. One male pup from each dam culled at weaning [postnatal day 21 (PND21)]. Results shown bodyweight in male offspring (M) from negative group dams (NG) had significantly heavier as compared to other pups groups. Total adipose tissue weight in MTX1 and MTX2 of the male offspring also show significantly lower compare to MNG. In mums, serum leptin of NG shows significantly higher as compared to CG group. Whereas, both treatment groups showed a significant reduction in serum leptin compared to NG group. In pups, MTX2 group show a more substantial reduction of body weight and serum leptin compared to other pups from other mums groups. In conclusion, E.tapos aqueous extract supplementation has a greater effect on ameliorating maternal obesity effects on male offspring by lowering body weight, inhibit fat deposition, and reducing serum leptin