Events The 1st International Electronic Conference on Microbiology

Event submissions

Published

10.3390/ECM2020-07096

This submission belongs to the session S1. Gut Microbiota and Health Disease of the event The 1st International Electronic Conference on Microbiology

Published date

02 Nov, 2020

Citation

Paulina Pecyna, Zuzanna Babalska, Klaudia Szymendera, Marcin Gabryel, Dorota Mańkowska-Wierzbicka, Dorota Nowak-Malczewska, Hanna Tomczak, Monika Górna, Małgorzata Chmielewska, Marcelina Maria Jaworska, Marian Grzymisławski, Agnieszka Dobrowolska, Marzena Gajęcka, QUALITATIVE IDENTIFICATION OF ROSEBURIA HOMINIS IN FAECES SAMPLES OBTAINED FROM PATIENTS WITH IRRITABLE BOWEL SYNDROME AND HEALTHY INDIVIDUALS, in Proceedings of The 1st International Electronic Conference on Microbiology, 2 November–30 November 2020, MDPI: Basel, Switzerland, doi: 10.3390/ECM2020-07096

Facebook

Twitter

QUALITATIVE IDENTIFICATION OF ROSEBURIA HOMINIS IN FAECES SAMPLES OBTAINED FROM PATIENTS WITH IRRITABLE BOWEL SYNDROME AND HEALTHY INDIVIDUALS

Paulina Pecyna ¹

Zuzanna Babalska ²

Klaudia Szymendera ²

Marcin Gabryel ³

Dorota Mańkowska-Wierzbicka ³

Dorota Nowak-Malczewska ²

Hanna Tomczak ^2,4

Monika Górna ²

Małgorzata Chmielewska ⁵

Marcelina Maria Jaworska ²

Marian Grzymisławski ³

Agnieszka Dobrowolska ³

Marzena Gajęcka ^2,6

1. Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland, Poland

2. Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland

3. Department of Gastroenterology, Dietetics and Internal Diseases, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland

4. Central Microbiology Laboratory, H. Swiecicki Clinical Hospital at the Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland

5. Chair and Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznan, Poland

6. Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznan, Poland

Abstract

Introduction: All the microorganisms inhabiting the particular human body regions or organs are collectively called a microbiome. The human body encompasses several various microbiomes that include specific populations of microorganisms. Gut microbial imbalance (dysbiosis) may lead to various diseases, including irritable bowel syndrome (IBS). While human microbiomes are abundant in non-breeding species, and traditional research methods based on classical microbiology have been proved to be ineffective, it is necessary to characterize in detail their composition to further evaluated function of particular microbiota. The knowledge about the microbial components of the intestinal ecosystem is still very limited, including Roseburia hominis.

Material and methods: The aim of the study was to identify Roseburia hominis in the faeces samples using PCR techniques. The study was conducted on samples obtained from patients with IBS (women, n=70, and men, n=50) and individuals without any intestinal symptoms (women, n=28, and men, n=23). After bacterial DNA extraction, qualitative identification of Roseburia hominis, based on the amplification of RHOM_14625 and RHOM_14635 gene fragments was performed. Then, a statistical analysis of the obtained data, using the Chi-square test was conducted.

Results: Assessed RHOM_14625 gene fragments were recognized in samples derived from 9 female and 21 male IBS patients, and in 15 and 7 female and male control individuals, respectively. The presence of the RHOM_14635 gene fragment was detected in 35 and 33 samples obtained from female and male patients, respectively, and in 18 and 7 samples from female and male control individuals. Differences in the analyzed genes distribution were statistically significant (p-value = 0.0001, and p-value = 0.02, respectively).

Conclusion: Further molecular studies are necessary to evaluate the role of Roseburia hominis in the intestinal microbiome in IBS patients.

Funding: Grant Preludium - NCN 2016/21/N/NZ5/01423 (principal: Paulina Pecyna).

Keywords

gut microbiome

gut microbiota

irritable bowel syndrome

Roseburia hominis

Manuscript

034200.docx.pdf

Future climate alters pathogens-microbiome co-occurrence networks in wheat straw residues during decomposition

Catabolism of branched chain and aromatic amino transferases, route to fusel alcohols and acetates by the Ceratocystidaceae.