Exploring the core microbial commonalities of alcoholics and CRC patients’

Understanding the link between the effect of lifestyle on gut microbiome and health status of an individual is one of the biggest scientific challenges in this decade. Our main goal was to find the commonalities between alcoholism and colorectal cancer (CRC) by evaluating the host gut microbiome. The microbial profiles of CRC patients were compared with the microbial findings of our study to establish alcoholism linked bacterial markers responsible for CRC development. A dataset of 504 colorectal cancer patients and CRC induced mice gut bacterial profiles were compared with the alcoholic volunteers and alcohol treated mice gut bacterial profiles, to find out the bacterial biomarkers specific for CRC. The core microbial profiles were evaluated using the raw fastq files of the previously reported data in both QIIME and MicrobiomeAnalyst pipelines. Interestingly, Bifidobacterium, Ruminococcaceae and Prevotella were found to be depleted (p≤0.01) in both CRC and alcoholic groups, while Clostridiales, Porphyromonadaceae, Bacteroides, Helicobacter, Lactobacillus, Klebsiella were found to be elevated compared to the healthy individuals (p≤0.01). The finding may serve as a key role in both early-stage detection and therapeutic intervention in the pathophysiological effect of alcohol leading to colorectal cancer development.