Spectral studies of the complex of adenosine with alcohol sugars

Our previous studies have shown the possibility of the formation of oligoribonucleotides (ORNs) complexes with alcohol sugar (D-mannitol (D-M)). A mixture of ORNs and D-M in a weight ratio of 3 to 1 has antiviral activity. ORNs consists of sequences of ribonucleotides of monophosphates. Therefore, we decided to investigate whether ORNs components form complexes with alcohol sugars. Adenosine is the major contributor to the ORNs emission spectra. Therefore, we have started studies on adenosine.

In the course of our work, the spectral characteristics of aqueous solutions of the sodium salt of adenosine and their mixtures with alcohol sugars (D-M, sorbitol, maltitol, and lactitol) studied. We have measured absorption, fluorescence, and excitation spectra of room temperature.

In the absorption spectra, a mixture of adenosine and alcoholic sugar did not make a significant difference compared to adenosine, as was the case with ORNs. Nevertheless, in the fluorescence spectra, we obtained significant differences between the fluorescence of adenosine and adenosine + alcohol sugars. We obtained the following changes:

Increasing the area of the excitation and radiation spectra with a shift of the position of the maximum of one radiation center and the other not.

Changing the energy distribution of the energy level diagram.

When adding other alcohol sugars, we have similar spectral changes, such as a shift in the maximum or a change in the shape of the peak, but have completely different effects of increasing or decreasing the intensity of the emission of its own fluorescence.