Events6th International Electronic Conference on Medicinal Chemistry
Event submissions
Published
with-doi10.3390/ECMC2020-07429
This submission belongs to the session C. General: Presentations of the event 6th International Electronic Conference on Medicinal Chemistry
Published date
06 Nov, 2020
Citation
Ivana Nikolic, Jovan Lukovic, Marina Mitrovic, Zoran Ratković, Jovana Muškinja, Marija Anđelković, Marina Živić, Tanja Zečević-Luković, Vanillin-based chalcone analogue induces cell death in HeLa and HCT-116 cell line through mitochondrial apoptotic pathway, in Proceedings of 6th International Electronic Conference on Medicinal Chemistry, 1 November–30 November 2020, MDPI: Basel, Switzerland, doi: 10.3390/ECMC2020-07429
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Vanillin-based chalcone analogue induces cell death in HeLa and HCT-116 cell line through mitochondrial apoptotic pathway

Jovan Lukovic 1
Marina Mitrovic 2
Zoran Ratković 3
Jovana Muškinja 4
Marija Anđelković 1
Marina Živić 5
Tanja Zečević-Luković 6
Ivana Nikolic 7
1. University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Biochemistry; Svetozara Markovica 69, 34000 Kragujevac, Serbia
2. University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Biochemistry
3. Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia
4. Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia
5. Dom Zdravlja, Čajetina, Serbia
6. University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Physical medicine and rehabilitation; Zmaj Jovina 30, 34000 Kragujevac, Serbia
7. University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Biochemistry; Svetozara Markovica 69; 34000 Kragujevac, Serbia
Abstract

Application of organic heterocyclic compounds as anti-tumor therapeutics are limited due to therapeutic drawbacks including resistance of tumor cells, non-selectivity of the administered drug towards healthy cells and abundance of unwanted side effects. The aim of our research was to investigate and compare both the antitumor effect and the mechanism of action of newly synthesized, so far untested, chalcone analogue on HeLa, HCT-116 cells and healthy human fibroblast lung cell line (MRC-5). The antitumor efficiency of investigated chalcone analogue was compared to the antitumor effects of dehydrozingerone and cisplatin that were used as referent substances. Cytotoxic and apoptotic effect were evaluated using both MTT test and flow cytometry by Annexin V-FITC/7-AAD staining. The results of our investigation indicated that newly synthesized chalcone analogue H5 expressed more powerful antitumor effect compared to the effects of both dehydrozingerone and cisplatin. Cell death was mediated via apoptotic pathway.

Keywords
apoptosis
cell death
chalcone
Oral Presentation
2020-06-03_1.Electronic_Conference_on_Medicinal_Chemistry Ivana Nikolic.pptx
Poster
2020-06-03_1.Electronic_Conference_on_Medicinal_Chemistry Ivana Nikolic.pdf
2020-06-03_1.Electronic_Conference_on_Medicinal_Chemistry Ivana Nikolic.pdf
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