Discrimination of Acne Vulgaris with Human Scalp Hair tissues using - FTIR-ATR Spectroscopy

Discrimination of Acne Vulgaris with Human Scalp Hair tissues using - FTIR-ATR Spectroscopy

Padmavathi R^1*, Rajamannan B² , Gunasekaran S³, Ramkumar GR⁴, Sankari G⁵_, Muthu S⁶

1 Department of Physics, Meenakshi Sundararajan Engineering College, Kodambakkam, Chennai, 600024,TN, India.

2 Engineering physics, FEAT Annamalai University, Annamalai Nagar, 608002, Chidambaram, TN, India.

3 Research and development St. Peter’s institute of Higher Education and Research, St. Peter’s University, Avadi,

Chennai, 600054, TN, India.

4 Department of Physics, C. Kandaswaminaidu College for Men, Chennai-600102, India.

5 Department of Physics, Meenakshi College for Women’s, Kodambakkam, Chennai-600024, India.

6 Department of Physics, Govt. Thirumagal Mills College, Gudiyatham-632602, Vellore, TN, India.

Correspondence; pathmavati@gmail.com

Abstract: Acne vulgaris is a chronic skin disease, which occurs due to inflammation of the hair follicles and sebum-producing (sebaceous) glands of the skin called pilosebaceous unit and the anaerobic propionic acne bacterium, P. Acne. Human sebum is dominantly made up about 57.5% of triglycerides and fatty acids, 26%wax esters, 12% Squalene, and 4.5% Cholesterol. The increased level Androgen hormone, sebum lipid composition, P. Acne overgrowth which induces monocytes and pro-inflammatory cytokines attract neutrophils, basophils, and T cells to the pilosebaceous unit and drive epithelial hyperproliferation i.e., Acne vulgaris. The actual Biomolecular changes due to acne vulgaris disease are present in the blood, in the sebum, and in the noninvasive sample of human scalp hair follicles. The main objectives of the present study are to analyze human scalp hair follicles samples using FTIR-ATR spectroscopy to compare and discriminate the spectral signatures of acne vulgaris and healthy scalp hair samples through acne biomarkers Protein, Amide I, Amide II and Squalene (LDL), using the method of internal ratio parameters. This work represents a first step in the development of the analytical tool for future drug development.

Keywords: Acne Vulgaris, Hair tissue samples, Discrimination, FTIR-ATR.