Sequence-Related Amplified Polymorphism (SRAP) Markers: A Feasible Tool for Studies in Invasion Genetics of Freshwater Fish ^†

Molecular tools can effectively be used in diagnosis, identification, management, and most especially detection of invasion pathways and originations of introduced species. It can provide helpful information for a better conception of the events that are essential for successful colonization by comparing genetic differentiations of a species within its native and invasive habitats. PCR-based dominant marker techniques, such as RAPD, AFLP and ISSR have been used for studying genetic diversity, taxonomy and phylogeny of the species, but they generally are used for estimating more limited levels of genetic variation. Despite the potential of these molecular markers to generate helpful data, researchers have been suspicious about using them resulting from technical deliminations. A more recently developed molecular marker system, Sequence-Related Amplified Polymorphism (SRAP) is an easy, reliable, middle-yield, high-dominant total, and iterative way to detect genetic variation of different species. SRAP markers were essentially created for molecular tagging in wild cabbage to particularly amplify coding regions of the genome. It also has been used in analyses of genetic variation and population genetics of several species such as fungi and nematodes. Like other dominant markers, SRAPs can also be used for assessing invasion genetics of non-native species nd their use to discover variations in genetic structure of native and invasive freshwater fish has been becoming common. Based on the rapidly growing body of literature, we suggest that SRAPs could, and should, be applied to study invasion genetics. The aim of this study was to identify the potential for SRAP markers in invasion genetics of freshwater fish. Results from SRAP products may prove to be significant for discovering polymorphisms and genetic variations in studying population genetics of invasive species.