Events The 1st International Electronic Conference on Antibiotics

Event submissions

Published

10.3390/ECA2021-09530 (registering DOI)

This submission belongs to the session A. Antimicrobial Resistance Mechanisms and Intrinsic Microbial Factors Contributing to Resistance of the event The 1st International Electronic Conference on Antibiotics

Published date

26 Mar, 2021

Citation

Isabel Carvalho, José António Carvalho, Ana Paula Castro, Gilberto Igrejas, Carmen Torres, Patrícia Poeta, Genetic diversity among selected ESBL and Carbapenem-producing Klebsiella pneumoniae isolates from urocultures in a portuguese hospital, in Proceedings of The 1st International Electronic Conference on Antibiotics, 8 May–17 May 2021, MDPI: Basel, Switzerland, doi: 10.3390/ECA2021-09530

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Genetic diversity among selected ESBL and Carbapenem-producing Klebsiella pneumoniae isolates from urocultures in a portuguese hospital

Isabel Carvalho ^1,2,3,4,5

José António Carvalho ⁶

Ana Paula Castro ⁶

Gilberto Igrejas ^2,3,4

Carmen Torres ⁵

Patrícia Poeta ^4,7

1. Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás‐os‐Montes and Alto Douro, Vila Real, Portugal, Portugal

2. Department of Genetics and Biotechnology, UTAD, Vila Real, Portugal

3. Functional Genomics and Proteomics Unit, UTAD, Vila Real, Portugal

4. Laboratory Associated for Green Chemistry (LAQV‐REQUIMTE), New University of Lisbon, Monte da Caparica, Portugal

5. Area Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain

6. Medical Center of Trás-os-Montes e Alto Douro E.P.E., Vila Real, Portugal

7. Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás‐os‐Montes and Alto Douro, Vila Real, Portugal

Abstract

Introduction and Objectives: Klebsiella pneumoniae is a major pathogen implicated in nosocomial infections that is known to spread easily, and it is frequently associated with resistance to the highest-priority critically important antimicrobials. The purpose of this work was to determine the genetic diversity (MLST) among selected carbapenem-and ESBL-producing K. pneumoniae isolates from human urinary infections.

Materials and Methods: Forty-nine cefotaxime/ceftazidime-resistant K. pneumoniae isolates were obtained aleatory from urocultures from patients of a Portuguese hospital during June 2017-July 2018. Identification was performed by MALDI-TOF MS. Antimicrobial susceptibility against 13 antibiotics was analyzed by disk diffusion test. Detection of ESBLs and other resistance and integron genes, and molecular typing (for selected isolates) was performed by PCR/sequencing.

Results: ESBL-production was detected in 26.5% of the isolates (13/49), most of them associated with CTX-M-15 enzyme (n=10). It is important to note that all ESBL-positive and negative isolates carried the KPC_2/3gene. Regarding the ESBL-producing Klebsiella pneumoniae isolates, different sequence types (ST) were identified (ST/phylogenetic-group/β-lactamase): ST15/CTX-M-15, SHV-28; ST15/CTX-M-15, SHV-12; ST280/CTX-M-15, SHV-27; ST280/SHV-27 and ST147/SHV-12. Additionally, the selected ESBL-negative isolates were typed as ST15/SHV-28, ST34/SHV-26 and ST348/SHV-11.

Conclusion: These findings indicate the genetic diversity among urinary infections isolates in our hospital. Furthermore, the KPC_2/3 is the main mechanism of carbapenem resistance in K. pneumoniae isolates, frequently associated with CTX-M-15 enzyme.

Keywords

Antibiotic resistance

Klebsiella pneumoniae

Carbapenems

Beta-lactamases

ESBL

Manuscript

Poster ECA 2021 - Isabel Carvalho final .pdf

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