EFFICIENT EXTRACTION AND STRUCTURAL ANALYSIS OF BIOSOURCED PIPERIN AS NATURAL ADJUVANT.

Introduction: In the framework of valorization of introduced and cultivated spices on Algerian soil, Piperin main constituent of black pepper is chemically and biologically studied, as it improves bioavailability of several synthetic and natural drugs such as resveratrol and Curcumin thanks to its diffusion mechanism and a high permeability coefficient, in fact it was reported that curcumin- Piperin nanoparticals were used to increase Curcumin bioavailability in cancers treatment.

Material and methods: In the present work an efficient Soxhlet extraction of Piperin with several solvents namely: ethanol, chloroform, dichloromethane, acetate ethyl, acetone; and time depending is reported in order to optimize extraction conditions and maximize extraction yields, besides a purification and structural characterization of obtained biocompounds was conducted using several analytical and spectroscopic methods as: MP, CCM, UV, FT-IR.

Results: Optimized Sohxlet extraction exhibits ethanol in 2h as the best solvent and time extraction conditions, and LC chromatography isolation in addition to spectroscopic analysis lead to identify target pure piperin.

Conclusion: The scope of this study is to use the obtained biobased Piperin in further applications like hemi synthesis or simply encapsulated and used as nutraceutical adjuvant to optimize efficiency of other biomoleculs.