ROOTING AND ADAPTATION OF SOLANUM TUBEROSUM L. UNDER EX VITRO CONDITIONS AFTER EXPOSURE TO DIFFERENT SUCROSE CONCENTRATIONS IN THE IN VITRO NUTRIENT MEDIUM

The microclonal propagation of potatoes is one of the most effective methods of biotechnology in breeding, since through callus genesis it provides ample opportunities for using various selective factors to induce somaclonal variability. The goal of our research is the problem of the influence of the composition of nutrient media, in particular sugar, on the engraftment of planting material in the in vitro potato culture in the soil.

Six potato varieties were used as the object of the research: Riviera (Netherlands), Kiranda (China), Plushka (Ukraine), Studentska (Ukraine), Hrenada (Ukraine), Kniazhna (Ukraine), and fragments of the apical meristem of potato plants were selected for in vitro introduction into the culture. Murashige and Skoog media (1962) with sucrose content modifications of 30 and 60% were used to induce callus genesis, while the control variant of the medium was 10% sucrose. The results of the research revealed a positive but uneven effect on the nature of rooting of in vitro culture plants of the varieties involved in the study. The promising options of potato varieties of different ripeness groups for further reproduction and use in the breeding process, which were grown on a medium with 10%, 30% and 60% sucrose content, were identified.