Objective: The objective of the present study aimed at investigating the phytochemical and Anti-microbial activity, Antioxidant and Anti-inflammatory properties of Carica papaya and Psidium guajava leaf extracts.

Methods: As phytochemicals are biologically active compounds and a powerful group of plant chemicals, believed to stimulate the immune system The present study focuses on the alcoholic and aqueous extraction of Carica papaya and Psidium guajava leaf extracts. The antioxidant activity has been studied in-vitro by using DPPH, and hydrogen peroxide assay. The test micro-organisms, gram negative (Klebsiella pneumonia and Escherichia coli) and gram positive (Staphylococcus aureus and Bacillus subtilis) bacteria were obtained. The results showed that aqueous and alcoholic leaf extracts possess antibacterial activity against tested organisms. The antidiarrheal effects of both aqueous and alcoholic leaf extracts extracts of the leaves of of Carica papaya and Psidium guajava were studied in rats against castor oil-induced diarrhea at the doses of 125 and 250 mg/kg body weight.

Results: The results of phytochemical screening revealed the presence of bioactive compounds such as alkaloid, carbohydrates, and amino acids varied in the different solvent extracts, in which alcoholic extracts showed good amount of phytochemicals and shows good Anti-microbial activity, Antioxidant and Anti-diarraheal activities. The alcoholic extract, and to a lesser extent the aqueous extract, significantly prolonged the time for diarrheal induction; it reduced the frequency of diarrheal episodes and decreased the propulsion of charcoal meal through the gastrointestinal tract.

Conclusion: The isolation and purification of specific bioactive compound may account as natural and promising medicines in exploration of new drug.