A rising threat of the rapid spread of acquired metallo-beta-lactamases (MBLs) among major Gram-negative pathogens is a matter of public health concern worldwide. The current study was undertaken to investigate the prevalence of Metallo-beta-lactamase producing gram-negative bacteria isolated from chicken meat in Bharatpur. The study was conducted for the period of 3 months from April 2023 to June 2023 in the Microbiology laboratory of Balkumari College, Bharatpur, Chitwan. Among the 40 collected sample, 12 (30%) isolates were E. coli, 10(25%) Citrobacter ,8(20%) Salmonella spp., 4(10%) Proteus spp., 4(10%) Shigella spp. and 2(5%) Klebsiella spp. Out of 12 isolates of E. coli species 10 (83.34%) isolates were found Multidrug resistance and 8 isolates were MBL positive. Out of 10 isolates of Citrobacter species, 7(70%) were found Multidrug resistance and 9 isolates were MBL positive. Similarly, out of 8 isolates of Salmonella species, 6(75%) were found to be Multidrug resistance and 5 isolates were MBL positive. Among the 4 isolates of Proteus species, 2(50%) were found to be Multidrug resistance and 3 were MBL positive. Out of 4 isolates of Shigella species, 3(75%) isolates were found to be Multidrug resistance and 3 isolates were MBL positive. Likewise, out of 2 isolates of Klebsiella species, 1 was found to be Multidrug resistance and both isolates were MBL positive.