The purpose of this study was to optimize the solid-state fermentation process of the stems and leaves of Angelicae Pubescentis Radix (APR) by probiotics, and to improve the osthole content in the stems and leaves of APR. In this experiment, Bacillus amyloliquefaciens SSYB was used to ferment the stems and leaves of APR. On the basis of a single-factor test, the solid-state fermentation process was optimized by an orthogonal test design, and the osthole content in the stems and leaves of APR before and after fermentation was determined by high-performance liquid chromatography. The results showed that the optimized fermentation process of the stems and leaves of APR was a fermentation time of 72 h, a fermentation temperature of 37°C, an inoculation amount of 5%, and 65% water content. Under this condition, the osthole content in the stems and leaves was 1.81 mg/g. It was significantly increased by 45.97 % (P<0.01) compared with that before fermentation. The results showed that the osthole content in the stems and leaves of APR was increased by optimizing the solid-state fermentation process of Bacillus amyloliquefaciens SSYB.