Pharmaceuticals in aquatic environments are a global concern, driving the search for sustainable removal technologies. Bioremediation is promising due to its environmental compatibility and low cost. Bacillus species are attractive candidates because of their metabolic versatility, resilience, and ability to remove emerging contaminants. However, improving removal efficiency remains essential for wastewater treatment.

This preliminary study evaluates the potential of Bacillus pumilus to remove ibuprofen, carbamazepine, diclofenac, and naproxen from contaminated water, supporting future optimization studies of this bacterial system.

The bacterium was cultivated in modified Marchlewicz et al. (2016) medium at 30 °C and 200 rpm, starting at an OD600 of ~0.03. Batch assays were performed with and without glucose (1 g L⁻¹) in 200 mL cultures containing individual pharmaceuticals (2–8 mg L⁻¹) or a mixture of the four compounds (2 mg L⁻¹ each) for 50 h. Growth was monitored by OD600 and residual pharmaceutical concentrations were quantified by HPLC–UV/Vis.

Controls with heat-inactivated cells and without glucose were included to assess adsorption onto bacterial biomass.

Culture medium composition affected growth, with glucose as an essential carbon and energy source. B. pumilus grew with all pharmaceuticals, although inhibition increased with concentration, and was strongest for ibuprofen (8 mg L⁻¹).

Pharmaceutical removal occurred under all conditions, reaching 50 h efficiencies of 6.8–11.5% (ibuprofen), 23–27% (naproxen), 10–15.5% (diclofenac), and 6.9–14.4% (carbamazepine). Removal was highest during the first 6 h of incubation. In fact, 25–88% of total removal occurred during the lag phase (2 h), when cell proliferation was minimal, indicating that adsorption onto bacterial biomass is the predominant initial mechanism, followed by slower removal during subsequent growth phases.

In summary, Bacillus pumilus removed the tested pharmaceuticals, with adsorption playing a major role in the initial phase. Although efficiencies were moderate, the results highlight its potential for pharmaceutical removal and support further optimization for wastewater treatment applications.

Marchlewicz A. et al., 2016. Water Air Soil Pollut. 227(6).