In the present scenario, nanotechnology is an important enabling technology which deals with the synthesis and control of matter at the molecular level, in scales smaller than 1 micrometer, normally 1 to 100 nanometers. Metal nanoparticles   are one of   the    most attractive   aspects of nanomaterials  in recent years. Synthesis of nanoparticles can be carried out by using various chemical and physical methods. But use of such methods is harmful in one or the other way as the chemicals often used are toxic, flammable. The purpose of this research work is to provide information on the recently discovered green synthesis process over conventional. This research opens an innovative design to improve antimicrobial activities. We did comparative study of synthesized silver and copper nanoparticles using unripe fruit of carica papaya extract. The papaya extract mediated synthesis of silver and copper nanoparticles was efficient and provides additional property such as bactericidal efficiency and might act as long searched alternative and could be answer to antibiotic resistance. The antibacterial activity of extracted biological synthesized silver and copper nanoparticle were observed against B. Substilis and E. Coli  using standard disc diffusion method. The green synthesis and characterization of  silver and copper nanoparticles were done and confirmed by spectroscopic method and FTIR technique.