The traditional systems of treatment such as Ayurveda, Unani, and Siddha, western herbal medicine, traditional Chinese medicine and homeopathy use herbs for the treatment. Many researchers has prescribed about the importance of herbal medicine in the treatment of various diseases and because of the accessibility and cost effectiveness herbal treatment is still in practice by large number of practitioners. Luffa cylindrica Linn. (Curcubitaceace ) fruits was reported to possess a number of medicinal properties including used as immunostimulant, anti-inflammatory, anti-tumour, antibacterial, antifungal and anti-viral activities and also induce uterine contraction to hasten child birth (Oxytocics). The present study was investigated the anti-ulcer activity of hydroalcoholic extracts of fruits of luffa cylindrica Linn by using aspirin plus pylorus ligated model at the dose of 100 and 200 mg/kg. The results were showed significantly (p<0.01) decreased in ulcer index (1.31±0.33) and gastric volume (2.18±0.74) when compared with control (3.92±0.27) and 95.85±0.12) respectively at the dose of 200 m/kg and also showed significant effect when compared with omeprazole (standard) at 2mg/kg. In this study concluded that the hydroalcoholic extract of fruit of luffa cylindrica act as anti-ulcer activity due to presence of alkaloids and flavonoids.