Isolation of Erwinia amylovora Bacteriophage from Soil and Pear Trees Plant Tissues
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Laboratory of Microbial Biotechnology, Enzymatics and Biomolecules (LBMEB), Center of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour km 6, PO Box 1177 Sfax 3018, Tunisia.
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Laboratory of Microbial Biotechnology and Engineering Enzymes (LBMIE), Center of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour km 6, PO Box 1177 Sfax 3018, Tunisia.
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Laboratory of Microbial Biotechnology and Engineering Enzymes (LBMIE), Center of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour km 6, PO Box 1177 Sfax 3018, Tunisia.
Astrum Biotech, Business incubator, Center of Biotechnology of Sf
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OMICS Research Group & Facility: Department of Biotechnology, Faculty of Applied & Computer Sciences, Vanderbijlpark Campus, Private Bag, X021 - Vanderbijlpark - 1911 - Andries Potgieter Blvd - South Africa
Published:
16 December 2018
by
MDPI
in
MOL2NET'18, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 4th ed.
congress
CHEMBIOMOL-04: Chem. Biol. & Med. Chem. Workshop, Paraiba, Porto, Rostock, Germany-Galveston, Texas, USA, 2018
Abstract: Attributed to a Gram-negative bacterium, identified as Erwinia amylovora, the fire blight disease, recently detected in Tunisia, has become a real threat to our economy and our arboriculture.
There is no effective curative treatment to eliminate the installed bacterium. It is therefore essential to prevent attacks and to limit the spread of the bacteria if it is already present. To do so we have chosen the biocontrol by bacteriophages.
In this work we have isolated new strains of Erwinia amylovora (10 strains) from plant tissues where fire blight symptoms are persistent. These strains have been the subject of a molecular study.
The isolated phages (30 isolates) were studied for their infectivity on Erwinia amylovora isolates and showed a surprising effect. Our phage isolates formed plaque of different sizes, with a diameter ranging between 0.5 and 2mm on the soft agar layer containing the test bacterium. The phages thermostability showed that all these viruses resist at 80 ° C for 45 min
Keywords: Erwinia amylovora, bacteriophage, soil, plant tissues, thermostability.
