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Preliminary phytochemical analysis and in vitro anti-cancer screening of ethanolic extract of roots of Bauhinia tomentosa L.against HUH-7 human liver cancer cell lines
Ramaiah Maddi
1  Professor & Head, Department of Pharmacognosy, Hindu College of Pharmacy, Amaravathi Road, Guntur - 522002, A.P., India

Published: 30 October 2019 by MDPI AG in 5th International Electronic Conference on Medicinal Chemistry session ECMC-5
10.3390/ECMC2019-06275
Abstract:

The most effective way to reduce the worldwide burden of liver cancer is to prevent it from happening in the first place. The current treatment of liver cancer has significant side effects. Hence, there is a need to develop anti-liver cancer agents of plant origin, which are less toxic, more efficacious and cost-effective. The present study has been performed experimentally by in vitro to examine the anti-liver cancer activity of roots of Bauhinia tomentosa L (Fabaceae). The roots of B. tomentosa was tested for its anti-cancer activity against HUH-7 human liver cancer cell lines by MTT assay. The standard used in this assay was Camptothecin (CPT) at 25µG concentration. Plant extract was tested at 25μg/mL, 50 μg/mL,100μg/mL, 200 μg/mL and 400 μg/mL concentrations. The percent cell viability of standard drug was found to be 49.59% and plant extracts at 25 μg/mL, 50 μg/mL,100 μg/mL, 200 μg/mL and 400 μg/mL concentrations were found to be 93.82%, 86.21%, 74.48%, 63.04%, 45.71% respectively. The cell morphology was observed and recorded under a microscope. The results clearly indicated that B. tomentosa shows a dose-dependent activity and it was maximum at 400μg/mL concentration where it shows 45.71% of liver cancer cell viability and it was comparable to the standard drug where it shows 49.59% of viability.

Keywords: Bauhinia tomentosa L, Roots, MTT Assay, HUH-7 human liver cancer cell lines, Anti-liver cancer
Comments on this paper
Wan Nor I'zzah Wan Mohamad Zain
Concentration of sample
Dear Professor Ramaiah Maddi,
Interesting research. I just would like to get your opinion on the sample concentrations. I noticed that the concentrations that you used were from 25-400 ug/mL. There were few conferences that I attended before and the judges were saying the range of concentrations of crude extract that we should use to screen the anticancer properties are up to 100ug/mL. More than that is considered too high as a potential anticancer drug.
What do you think about this? Thank you in advance for your explanation.
Dr Ramaiah Maddi
Dear Wan Nor I'zzah Wan Mohamad Zain
Thank you for your interest.
Yes I agreed for the same. The concentration generally used upto 400ug/mL depends on the plant specific and varies from one species to another, also depends on other parameters like geography, time of cultivation and collection. Some cases we can consider.


 
 
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