The research activity is aimed at the development and validation of chromatographic methods for the qualitative and quantitative determination of biologically active molecules in human and animal, cosmetics, food, and environmental complex matrices. These procedures have been applied to different analytes and drugs/drug associations, also finding application in clinical and pre-clinical studies, to characterize new delivery systems of the active principle to improve their pharmacological properties. In the development of the method, predictive models and chemometrics are applied for both the optimization of extraction protocols and for final data processing. Particular attention is given to the innovative and green (micro) extraction techniques and new instrumental configurations for complex matrix quantitative analysis. Scientific activity is proven by more than 320 publications in International peer-reviewed Journals, by 30 book chapters and 10 books, and by more than 220 oral and poster communications at National and International conferences.