Shrimp aquaculture in Malaysia has expanded to meet the increasing demand for food commodities, mainly focusing on whiteleg shrimp (Litopenaeus vannamei). The shift towards intensive farming systems has been adopted to enhance productivity. However, these high-density farming practices elevate the risk of disease outbreaks, notably from pathogenic bacteria such as Vibrio vulnificus. This Gram-negative bacterium is associated with serious health issues, including gastroenteritis, which can lead to severe wound infections or fatality. It also poses a substantial public health risk due to the emergence of multidrug resistance. Therefore, this study aimed to determine the prevalence rate and quantify the presence of V. vulnificus in 67 samples of whiteleg shrimps sold at wet markets and hypermarkets in Sri Kembangan, Malaysia. Several methods have been used, including isolation and detection on CHROM agar and MPN-PCR. Furthermore, antibiotic susceptibility tests were performed using ten types of clinical antibiotics by applying the standard disk diffusion method. The overall prevalence rate of V. vulnificus in whiteleg shrimp was 40.30% (28/67), consisting of 48.15% (13/27) from the hypermarket and 51.85% (14/27) from the wet market. The total coliform count in the samples reached 4.6 MPN/g, which exceeded the acceptable limit of 3 MPN/g set by the Malaysian Food Act 1983 and its associated regulations. Moreover, the t-test analysis indicated no significant difference in total coliform count between wet market and hypermarket samples. Antibiotic susceptibility tests revealed that 60% of V. vulnificus strains were susceptible to doxycycline, while 100% were resistant to amoxicillin. In conclusion, the results highlight significant concerns regarding the high bacterial contamination levels in the shrimp samples and the presence of antibiotic-resistant microorganisms. Hence, this study could increase awareness about the correct handling and storing of raw shrimp to minimize bacterial contamination and to be aware of the usage of antibiotics in farms to prevent further resistance development.