INTRODUCTION

In ancient times, silver played an important role in curing many diseases to fight against many infections. Now, silver is used as AgNPs for targeting many biomedical and physio-chemical reactions to fulfil research goals. But there are many drawbacks that have also been reported in reactions to AgNPs, like allergy and environmental risks, so to avoid these side-effects, plant-based AgNPs are synthesized by researchers. In our research, we have used silver nano-particles of Phyllanthus emblica and Adhatoda vasica Leaf Extract and their comparative study on microbes.

METHODS

Amla and adusa leaf samples were first collected, then crushed into a powder. Next, we made a water-based extract solution by heating the mixture to 80 degrees Celsius for three to four hours using a magnetic stirrer. Finally, the leaf extract was combined with 1M silver nitrate solution, which was made by dissolving 1.7 grams of silver nitrate in 100 milliliters of water. Finally, the mixture of amla and adusa silver nitrate was centrifuged at 12000 rpm for 30 minutes, discarding the supernatant and collecting the dark pellet to form AgNPs from the leaf extract. Finally, the leaf extract was collected in the form of a powder and dried for two to three days in a dark place. Using the disc diffusion and well diffusion methods, we investigated the effects of these AgNP powders at varying concentrations against bacteria that cause disease, such as E. Coli, S. Aureus, Mucor, and Aspergillus strains. Additionally, we utilized the commercial antibiotic streptomycin to complete a comparative study.

CONCLUSIONS

In our research, AgNPs of amla and adusa leaf extracts, comparatively studied on microbes, generated positive results as compared to commercial antibiotic streptomycin for killing microbes that were clearly shown by zone-of-inhibition formation on petriplates.