Introduction

Extremely low frequency (ELF) electromagnetic fields (EFs) represent an innovative meansof treating cancer. They affect various biological processes in cells, such as proliferation, metabolism, and cell cycle, which play a key role in the development of cancer cells. Intercellular interactions based on the EF regulate cell migration and morphogenesis. These processes are closely related to the function of the centrosome and intercellular communication. EFs can increase apoptosis and inhibit the angiogenesis and proliferation of tumor cells.

Methods

In order to investigate the effect of ELFs on the action of anticancer drugs, a device was developed that allows for the assessment of the effect on cell cultures on various cancer cell lines (including those taken from the patient) and the use of anticancer drugs in the appropriate concentration. It is possible to investigate the effect of the selected EF intensity in the ELF range to determine the optimal time of field exposure.

Results

The initial evaluation of device performance and the determination of EF parameters were performed on three human cancer cell lines (LoVo, MC7, and A431) treated with doxorubicin. In relation to the EF parameters, the operating range of the field with a frequency of 50 Hz and an induction of 1.25 mT was established. The EF distribution inside the solenoid was examined. Only cells within an area no greater than 5 cm from the central point of the solenoid may be considered as having been subjected to uniform exposure with non-uniformities not exceeding +10%.

Conclusion

The described method allows for an effective and fast way of checking the influence of EF on the pharmacological effect of the drug for a given type of cancer. This model can thus help in selecting the appropriate EF parameters, allowing for a reduction in the cytostatic dose while maintaining the effectiveness of the therapy.