Introduction:
The development of functional hydrogels through 4D printing represents a breakthrough in biomedical and pharmaceutical applications. By incorporating bioactive extracts from natural sources such as Rhodiola rosea (Różeńca górskiego) and Centella asiatica (Gotu Kola), these hydrogels offer enhanced therapeutic potential. The project investigates the chemical composition and properties of these plants and their extracts, aiming to utilize their bioactive compounds in hydrogel matrices.
Methods:
The chemical composition of Rhodiola rosea and Gotu Kola extracts was analyzed using spectroscopic and chromatographic techniques to identify key bioactive components. Hydrogels were synthesized using sodium alginate, hyaluronic acid, and poly(vinyl alcohol), and were 4D-printed using a customized extrusion-based printer. The bioactive extracts were incorporated during the hydrogel preparation, ensuring their stability and functionality.
Results:
The hydrogel matrices demonstrated high biocompatibility and stability, with controlled swelling and degradation profiles suitable for biomedical applications. Incorporation of Rhodiola rosea extract, rich in salidroside and rosavins, enhanced antioxidant and adaptogenic properties. Meanwhile, the inclusion of Gotu Kola extract, containing asiaticoside and madecassoside, significantly improved wound-healing and anti-inflammatory capabilities. The combined use of alginate, hyaluronic acid, and poly(vinyl alcohol) facilitated cross-linking, mechanical robustness, and sustained release of bioactive compounds.
Conclusion:
This study highlights the potential of 4D-printed hydrogels as advanced biomaterials for drug delivery and tissue engineering applications. The integration of bioactive plant extracts from Rhodiola rosea and Gotu Kola offers a novel approach to enhancing the functional and therapeutic properties of hydrogels, paving the way for innovative solutions in regenerative medicine and pharmaceutical formulations.
Acknowledgments: Acknowledgments: This research was carried out within the SMART-MAT Functional Materials Scientific Club of the Faculty of Materials Engineering and Physics at Cracow University of Technology and as part of the project entitled " Functional hydrogels obtained using 4D printing" financed by the FutureLab organization operating at Cracow University of Technology
