Piper methysticum, traditionally used in the Pacific for religious, medical, and social purposes, has garnered recent fame as a nutraceutical for anxiety treatment. Sansevieria trifasciata (snake plant) has antibacterial, cytotoxic, and wound-healing properties. The ethanolic extracts of both plants were studied for their chemical group, antimicrobial, and CNS depressant effects. The disc diffusion method was used to assess the antimicrobial activity of Piper methysticum and Sansevieria trifasciata. Their central nervous system (CNS) depressant effects were evaluated using classical depression models, including hole cross, elevated maze, forced swimming, and tail suspension tests in Swiss albino mice. Mice were divided into control, positive control, and three test groups, with doses of 250 mg/kg and 500 mg/kg for hole cross and elevated maze tests, and 50, 100, and 200 mg/kg for forced swimming and tail suspension tests. Diazepam was used as the standard drug. Phytochemical screening of both plant extracts revealed the presence of alkaloids, flavonoids, reducing sugars, steroids, saponins, terpenoids, and amino acids. Both ethanolic extracts exhibited strong antibacterial activity in disk diffusion testing. S. trifasciata was most effective against E. coli and S. cerevisiae, while P. methysticum showed significant antimicrobial activity against Shigella dysenteriae and Salmonella typhi. In the hole cross test, both extracts significantly reduced locomotor activity across all doses (P<0.01), indicating CNS depressant effects. However, neither extract showed significant changes in the number of entries or time spent in the open arms during the elevated maze test. Both extracts significantly reduced immobility time in the forced swimming and tail suspension tests (P < 0.01 and P < 0.001), with diazepam increasing immobility time (P < 0.001). These results suggest that both plant extracts have CNS depressive and antidepressant-like effects. Both the ethanolic extracts of P. methysticum and S. trifasciata demonstrate antimicrobial and CNS depressant effects. Further studies are needed to continue pharmacological research for their potential use in medicine.