In this study, optimized natural polymeric membranes were developed using two advanced manufacturing methods: solution casting and electrospinning. Both processes required exhaustive optimization to minimize visual and microscopic defects. The natural polymers used were polylactic acid (PLA) and (PLA+ lignin (9 %wt)), while acetone and dichloromethane were the solvents.
Next, the membranes were characterized using several surface analysis techniques. A confocal microscope and scanning electron microscopy (SEM) were employed to examine surface morphology (the diameter of the fibers was between 1.6 for PLA and up to 2.2 micrometers for the PLA+Lignin (9 %wt) fibers (27-35% standard deviation in both situations). Contact angle measurements were taken to assess wettability (between 90-100° for PLA and up to 140-150° for PLA+Lignin (9 %wt)), and UV-Vis spectroscopy was used to evaluate the optical transparency (the PLA+Lignin fiber membranes (9 %wt) were between 70 and 80% less transparent than the PLA fiber membranes).
The main objective of this study was to explore potential industrial applications. Due to the known antimicrobial properties of certain functional groups present in lignin, the membranes were subjected to antimicrobial testing. Two evaluation methods were employed, the Kirby–Bauer disk diffusion method and the direct contact method, following standardized protocols ASTM E2149, JIS Z 2801, and ISO 22196. Four bacterial strains were used: Staphylococcus aureus (Gram-positive), Escherichia coli (Gram-negative), Staphylococcus epidermidis (Gram-positive), and Micrococcus luteus (Gram-positive).
The results revealed notable antimicrobial activity, particularly against Micrococcus Luteus with electrospinning (PLA+Lignin (9 %wt)) membranes with direct contact, suggesting that these membranes could be promising candidates for biomedical applications.
This study demonstrates a sustainable strategy for fabricating nanostructured membranes with inherent antimicrobial properties, paving the way for future biomedical and packaging applications.
