The growing canine population in urban centers, such as Curitiba, Brazil—where there is approximately one dog for every three residents—has led to an increased demand for pet treats, including dehydrated animal by-products. While these products are widely accepted by pet owners, their microbiological quality remains underexplored, posing potential health risks to both animals and humans.This study aimed to evaluate the microbiological safety of dehydrated pig and cattle ears sold as dog treats. Twenty samples (10 bovine and 10 porcine ears) were collected from four commercial brands, grouped into four sets of five samples each from the same production batch. All samples were within the expiration date, with intact packaging and no visible alterations. Microbiological analyses were performed at the Quality Control and Food Safety Laboratory of the Federal University of Paraná, focusing on the enumeration of Total Coliforms, Thermotolerant Coliforms, yeasts, and molds. In 50% of the samples, none of the targeted microorganisms were detected. However, Total Coliforms were present in 25% (5/20), Thermotolerant Coliforms in 10% (2/20), and Molds and Yeasts in 35% (7/20) of the products. Contamination levels varied across brands but were found in both bovine and porcine samples. The presence of indicator microorganisms highlights the need for improved hygienic practices in the production and handling of these products. Such contamination poses not only a risk to animal health but also to human health through potential cross-contamination. The findings emphasize the importance of microbiological monitoring of pet treats to ensure food safety within a One Health framework.