Introduction and Aim
An efficient and eco-friendly synthetic approach was developed for the preparation of tetrahydropyrimidine carboxamide derivatives using a multicomponent reaction (MCR) strategy. This method offers advantages such as high yields, mild conditions, short reaction times, structural stability, and reusability of reagents. The synthesized 1,2,3,4-tetrahydropyrimidine carboxamides were evaluated for their antifungal, antibacterial, and antioxidant activities. Molecular docking studies were also performed to analyze their binding modes and interaction profiles with biological targets.

Materials and Methods
A novel series of 5-carboxamide-substituted 1,2,3,4-tetrahydropyrimidine-2(1H)-ones (DHPMs) was synthesized via MCR. Reaction conditions were optimized to enhance yield and reduce reaction time. Based on preliminary biological screening, selected compounds were subjected to molecular docking using known THPC inhibitors as references. These studies aimed to understand receptor-ligand interactions and support the in vitro findings.

Results
The synthesized THPC derivatives were characterized by IR, mass spectrometry, ¹³C NMR, and ¹H NMR spectroscopy. The compounds were evaluated for in vitro antioxidant, antibacterial, and antifungal activities. Antibacterial activity was tested against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa. Several derivatives exhibited significant antimicrobial activity, comparable or superior to standard drugs. Molecular docking revealed stable hydrogen bonding and π–π interactions with target proteins.

Conclusion
The synthesized tetrahydropyrimidine carboxamide derivatives, particularly those with heterocyclic substitutions, demonstrated potent biological activity. Molecular docking confirmed favorable interactions, supporting their potential as leads in drug development. This integrated approach provides a foundation for further structure–activity relationship (SAR) studies.