Natural Polysaccharides are polymers composed of monosaccharide units bound together by glycosidic linkages. In their native form, these polymers may not be able to provide all the desired properties for a particular application. Thus, the functionalization of its reactive hydroxyl groups would change their surface properties. In this work, we evaluated the derivatization of OH groups of maltodextrin polysaccharide with urea. By a one-pot selective procedure carried out at room temperature for 3 h, urea united at maltodextrin through covalent bonding was obtained. The results showed that after functionalization with urea, the pH and solubility in polar solvents of the new material increased. Derivatization of maltodextrin with urea confers stability, and terminal primary amine groups on the surface of this material represent a versatile reactive functional group for conjugation with other molecules for potential applications in the development of new materials.