Salinity resistance of the African rice species (Oryza glaberrima) is poorly documented and the specific responses of the plant to Na⁺ and Cl^- toxic ions remain unknown. Moreover, the roles of Na⁺/H⁺ antiport and cation-chloride-cotransporter in salt stresses still remain unclear. In 1^st experiment, two distinct cultivars TOG5307 and TOG5949 were maintained for 15 days on iso-osmotic nutrient solutions containing 50 mM NaCl, or a combination of Cl^- salts (Cl^- - dominant) or Na⁺ salts (Na⁺ - dominant). Plant water status, ion accumulation, gas exchange and fluorescence related parameters; carbon (∆13C) and nitrogen (δ15N) isotope discrimination were analyzed. TOG5307 exhibited a higher level of resistance than TOG5949 in terms of growth and photosynthesis maintenance and control of Cl^- and Na⁺ accumulation. NaCl was the most detrimental treatment, followed by Na⁺ -dominant treatment while Cl^- -treatment had the lowest effect. Impact of Na⁺ and Cl^- on considered parameters are additive. In 2^nd experiment, the two contrasted cultivars (TOG5307 and TOG5949) were exposed during 3 days in nutrient solutions to 75 mM NaCl containing 100 µM amiloride (inhibitor of Na⁺-H⁺ antiporters) or 200 µM bumetanide (inhibitor cation-chloride-cotransporters). Amiloride increased Na⁺ accumulation in roots and leaves to a higher extent in salt-resistant TOG5307 than in salt-sensitive TOG5949. Bumetanide reduced Cl^- accumulation in both cultivars as well as K⁺ accumulation in TOG5307 and Na⁺ accumulation in TOG5949. Cultivars exhibiting contrasting levels of salinity resistance are available in Oryza glaberrima and salt-tolerant genotypes may constitute a valuable source of gene for classical rice improvement.