Aflatoxin B1 (AFB1) contamination in food poses serious problems both for economic development and public health protection, thus leading to a focus on an effective approach to control it. In this context, probiotics strains (Lactobacillus kefiri KFLM3, Kazachstania servazzii KFGY7 and Acetobacter syzygii KFGM1) isolated from a Kefir culture were assessed for their AFB1 adsorption ability. The adsorption experiments were done in culture medium and in milk. The stability of microorganism/AFB1 complexes was evaluated using buffer solutions (pH=3, pH=7 and pH=8) to simulate the pH conditions in the gastrointestinal tract. Our results showed that strains binding assays for AFB1 in culture medium showed no effect (0%). However, the strain L. kefiri KFLM3 was the most active, adsorbing 80 % of AFB1 when cultivated in milk followed by A. syzygii KFGM1 (74%) and K. servazzii KFGY7 (65%). Nonetheless, the strain K. servazzii KFGY7 retained more AFB1 after the desorption experiments (65%). The present findings suggest that kefir isolated strains might be a promising candidate for exploitation in AFB1 detoxification in food and feed matrices.