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Comparison of The Performances of Two RNA-Based Geno-Sensing Principles for The Detection of lncPCA3 Biomarker
* 1 , * 1 , * 2 , * 3
1  Sheffield Hallam university, Materials and Engineering Research Institute, UK
2  Sheffield Hallam University, Biomedical Research Centre, UK
3  The University of Sheffield, Department of Chemistry, UK
Academic Editor: BenoƮt Piro

Abstract:

The most common prostate cancer (PCa) diagnostics which is based on detection of prostate-specific antigen (PSA) in blood has specificity limitations often resulting in both false-positive and false-negative results ; therefore, improvement in PCa diagnostics using more specific PCa biomarkers is of high importance. Studies have shown that the long noncoding RNA Prostate Cancer Antigen 3 (lncPCA3) over-expressed in the urine of prostate cancer patients is an ideal biomarker for non-invasive early diagnostics of PCa. Geno-sensors based on aptamer bio-receptors (Apta-sensors) offer cost- and time-effective, and precise diagnostic tools for detection PCa biomarker . In this study, we report on further development of RNA-based Apta-sensors exploiting two different detection strategies, i.e. electrochemical (CV and IS) and optical (spectroscopic ellipsometry) measurements. These sensors were made by immobilization of thiolated CG-3 RNA aptamers on the surface of gold. Aptamer labelled with redox group (ferrocene) was used in electrochemical measurements, while non-labelled aptamer was used in total internal reflection ellipsometry (TIRE) measurements. The results obtained by these two methods were compared, the sensitivity in FM level of concentration was achieved and the required selectivity is provided by high affinity of PCA3-to-aptamer binding with KA in 107 L/mol range. The conditions for the aptamer immobilisation procedure (aptamer concentration, incubation time) were optimised. The detection of PCA3 in urine was attempted. The proposed detection approaches allow the reliable detection of PCA3 at low concentrations, thus providing a background for future development of novel, highly sensitive and cost-effective diagnostic methodologies for prostate cancer detection.

References:

  1. Salman, J.W. et al., Prostate specific antigen as a tumor marker in prostate cancer: Biochemical and clinical aspects. In Advances in Cancer Biomarkers; Springer: 2015, 867, 93–114.
  2. Schmid, M. et al. Urinary prostate cancer antigen 3 as a tumour marker: Biochemical and clinical aspects. In Advances in Experimental Medicine and Biology; Springer, 2015; p. 291.
  3. Nabok A., Abu-Ali, H., Takita, S., Smith, D.P., Electrochemical detection of prostate cancer biomarker PCA3 using specific RNA-based aptamer labelled with ferrocene, Chemosensors, 2021, 9, 59.

Keywords: prostate cancer; PCA3; Geno-sensor; RNA aptamer
Comments on this paper
Magdi Mussa
well done, good proceeding
I think this approach of bioscinsor in cancer study is critical can open new horizons when compared to the traditional methods; well done



 
 
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